Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification

Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification

Blog Article

The recent cases of COVID-19 have brought the prospect of and requirement for point-of-care diagnostic devices into the limelight.Despite all the advances in point-of-care devices, CAYENNE 40 there is still a huge requirement for a rapid, accurate, easy-to-use, low-cost, field-deployable and miniaturized PCR assay device to amplify and detect genetic material.This work aims to develop an Internet-of-Things automated, integrated, miniaturized and cost-effective microfluidic continuous flow-based PCR device capable of on-site detection.As a proof of application, the 594-bp GAPDH gene was successfully amplified and detected on a single system.The presented mini thermal platform with an integrated microfluidic device has the potential to be used for the Console Bracket detection of several infectious diseases.